CLIP170 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36749

Novus Biologicals, part of Bio-Techne
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NBP1-36749
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CLIP170 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PHSTHHGSRGEERP corresponding to C-Terminus according to NP_937883.1.

Epitope

C-Terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CLIP170 Antibody

Western Blot: CLIP170 Antibody [NBP1-36749]

Western Blot: CLIP170 Antibody [NBP1-36749]

Western Blot: CLIP170 Antibody [NBP1-36749] - Analysis of CLIP170 in Human Breast Cancer lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749]

Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749]

Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749] - Staining of paraffin embedded Human Prostate. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749]

Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749]

Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749] - Staining of paraffin embedded Human Thyroid Gland. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CLIP170 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 160 kDa band observed in human breast cancer lysates (calculated MW of 161 kDa band according to NP_002947.1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLIP170

Cytoplasmic linker protein of 170 kDa (CLIP-170) is the original member of a group of microtuble binding proteins designated as plus-end-binding proteins(+TIPs). CLIP-170 binds to the growing plus ends of microtubules and acts as a linker between the dynamic microtubule ends and organelle membranes. The protein acts as an anticatastrophic factor, promoting microtubule rescue near the cell periphery. Fluorescently labeled CLIP-170 can be visualized as a comet like streak around the growing ends of microtubules. CLIP-170 colocalizes with dynactin and dynein at microtubule ends and also at the kinetochore. Restin, first identified as a marker for Hodgkin and Reed-Sternberg (HRS) cells, is a 160 kDa splice variant of the gene that includes a 35 amino acid stretch not present in CLIP-170. CLIP-170/restin is highly expressed in HRS cells, monocyte-derived dendritic cells, IL-4 + CD40L activated B cells, and in Ki-1 lymphoma.

Alternate Names

CAP-GLY domain containing linker protein 1, CLIP, CLIP170, CLIP-170Reed-Sternberg intermediate filament-associated protein, CYLN1MGC131604, cytoplasmic linker 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Steinberg cell-expressed intermediate filament-associated protein, Restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein), RSNCAP-Gly domain-containing linker protein 1

Entrez Gene IDs

6249 (Human); 56430 (Mouse); 65201 (Rat)

Gene Symbol

CLIP1

UniProt

NP_002947.1 (Human)

Additional CLIP170 Products

Product Documents for CLIP170 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CLIP170 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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