CLOCK Antibody (3D8H6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16656

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16656-100ul
NBP3-16656-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3D8H6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CLOCK Antibodies »

Product Summary for CLOCK Antibody (3D8H6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CLOCK (NP_004889.1). WKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLOCK Antibody (3D8H6)

Western Blot: CLOCK Antibody (3D8H6) [NBP3-16656]

Western Blot: CLOCK Antibody (3D8H6) [NBP3-16656]

Western Blot: CLOCK Antibody (3D8H6) [NBP3-16656] - Western blot analysis of extracts of various cell lines, using CLOCK Rabbit mAb (NBP3-16656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656]

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656]

Immunocytochemistry/Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656] - Immunofluorescence analysis of U-2 OS cells using CLOCK Rabbit mAb (NBP3-16656) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656]

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656]

Immunocytochemistry/Immunofluorescence: CLOCK Antibody (3D8H6) [NBP3-16656] - Immunofluorescence analysis of C6 cells using CLOCK Rabbit mAb (NBP3-16656) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for CLOCK Antibody (3D8H6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLOCK

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Long Name

Circadian Locomoter Output Cycles Kaput

Alternate Names

BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Gene Symbol

CLOCK

Additional CLOCK Products

Product Documents for CLOCK Antibody (3D8H6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CLOCK Antibody (3D8H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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