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CLOCK Antibody (8F7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51610

Novus Biologicals, part of Bio-Techne
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NBP1-51610
NBP1-51610-0.025ml

Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8F7

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human CLOCK expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLOCK Antibody (8F7) - BSA Free

Western Blot: CLOCK Antibody (8F7)BSA Free [NBP1-51610]

Western Blot: CLOCK Antibody (8F7)BSA Free [NBP1-51610]

Western Blot: CLOCK Antibody (8F7) [NBP1-51610] - Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (8F7) - BSA Free [NBP1-51610]

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (8F7) - BSA Free [NBP1-51610]

Immunocytochemistry/Immunofluorescence: CLOCK Antibody (8F7) [NBP1-51610] - Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Applications for CLOCK Antibody (8F7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLOCK

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Long Name

Circadian Locomoter Output Cycles Kaput

Alternate Names

BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Gene Symbol

CLOCK

Additional CLOCK Products

Product Documents for CLOCK Antibody (8F7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CLOCK Antibody (8F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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