CLOCK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-126

Novus Biologicals, part of Bio-Techne
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NB100-126
NB100-126SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CLOCK Antibodies »

Product Specifications

Immunogen

Two peptides derived from mouse CLOCK conjugated to albumin. [UniProt# O08785]

Localization

Cytoplasmic and Nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLOCK Antibody

Western Blot: CLOCK Antibody [NB100-126]

Western Blot: CLOCK Antibody [NB100-126]

Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.

Applications for CLOCK Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Western Blot

1:1000
Application Notes
This CLOCK antibody is useful for Western blot and Chromatin Immunoprecipitation. It has been tested against bacterially expressed fragment of CLOCK expressed in BL21 cells or the full length protein expressed in an in vitro translation system (reticulocyte lysate).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CLOCK

Different physiological as well as behavioral processes such as sleep-wake cycles, thermoregulation, hormonal secretions, blood pressure and metabolism exhibits 24-hour rhythmicity which is generated through a molecular machinery involving transcriptional-translational feedback loop in the suprachiasmatic nucleus (SCN) of hypothalamus and many peripheral tissues. Mammalian circadian system contains a set of core CLOCK genes that encode proteins such as CLOCK (circadian locomotor output kaput), BMAL1 (brain and muscle ARNLT1), PER (period), CRY (cryptochrome), REV-ERB alpha, and ROR alpha. CLOCK was the first gene identified in its family and CLOCK protein binds DNA for activating transcription after dimerization with BMAL1 by driving the rhythmic transcription of other CLOCK and circadian clock-controlled genes through an E-box (5'-CACGTG-3'). It activates transcription of PER1/PER2 which is inhibited in a feedback loop by PER and CRY proteins. With its intrinsic histone acetyltransferase activity, CLOCK acetylates histones H3, histone H4, and ARNTL (non-histone substrate), and has also been shown to plays a role in DNA damage response/DDR signaling during the S phase of cell cycle. Because this endogenous timekeeper interacts with countless biological systems, CLOCK dysfunctions may lead to the pathogenesis of several diseases including sleep disorders, metabolic syndrome, cardiovascular and inflammatory disease, and cancer.

Long Name

Circadian Locomoter Output Cycles Kaput

Alternate Names

BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Entrez Gene IDs

9575 (Human)

Gene Symbol

CLOCK

UniProt

O08785 (Mouse)

Additional CLOCK Products

Product Documents for CLOCK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CLOCK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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