CLOCK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88614
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TQDRQIRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQSQTLSVTQQQQQQSSQEQQLTSVQQPSQAQLTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPQSHHQQHQSQQQQQLSRHRTDSLPD
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CLOCK Antibody
![Immunocytochemistry/ Immunofluorescence: CLOCK Antibody [NBP1-88614]](https://resources.bio-techne.com/images/products/CLOCK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88614-img0005.jpg "Immunocytochemistry/ Immunofluorescence: CLOCK Antibody [NBP1-88614]")
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody [NBP1-88614]
Immunocytochemistry/Immunofluorescence: CLOCK Antibody [NBP1-88614] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.![Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614]](https://resources.bio-techne.com/images/products/CLOCK-Antibody-Immunohistochemistry-Paraffin-NBP1-88614-img0004.jpg "Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614]")
Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614]
Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -
Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -Staining of human duodenum shows strong nuclear and cytoplasmic positivity in glandular cells.Applications for CLOCK Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLOCK
CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.
CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.
Long Name
Circadian Locomoter Output Cycles Kaput
Alternate Names
BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein
Entrez Gene IDs
9575 (Human)
Gene Symbol
CLOCK
UniProt
Additional CLOCK Products
Product Documents for CLOCK Antibody
Product Specific Notices for CLOCK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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