CLPP Antibody (1W6O4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16156

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16156-100ul
NBP3-16156-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1W6O4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CLPP Antibodies »

Product Summary for CLPP Antibody (1W6O4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 178-277 of human CLPP (Q16740). HQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPAST

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLPP Antibody (1W6O4)

Western Blot: CLPP Antibody (1W6O4) [NBP3-16156]

Western Blot: CLPP Antibody (1W6O4) [NBP3-16156]

Western Blot: CLPP Antibody (1W6O4) [NBP3-16156] - Western blot analysis of extracts of various cell lines, using CLPP Rabbit mAb (NBP3-16156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156]

Immunocytochemistry/ Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156]

Immunocytochemistry/Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156] - Immunofluorescence analysis of NIH-3T3 cells using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156]

Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156]

Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156] - Immunohistochemistry of paraffin-embedded human colon using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for CLPP Antibody (1W6O4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLPP

CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

Alternate Names

ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli), ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli), EC 3.4.21.92, Endopeptidase Clp, putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Gene Symbol

CLPP

Additional CLPP Products

Product Documents for CLPP Antibody (1W6O4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CLPP Antibody (1W6O4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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