CLPP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1189

Novus Biologicals, part of Bio-Techne
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NB100-1189
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CLPP Antibodies »

Product Summary for CLPP Antibody

Immunogen

Peptide with sequence C-EAAPAAEPVPAST corresponding to C-Terminus according to NP_006003.1.

Localization

Mitochondrial

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CLPP Antibody

Western Blot: CLPP Antibody [NB100-1189]

Western Blot: CLPP Antibody [NB100-1189]

Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189]

Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189]

Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189] - IHC staining of CLPP in paraffin embedded Human Liver using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: CLPP Antibody [NB100-1189]

Western Blot: CLPP Antibody [NB100-1189]

Western Blot: CLPP Antibody [NB100-1189] - Analysis of CLPP in human muscle lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 1 ug/ml. Primary antibody incubated for 1 hour. Detected using chemiluminescence.

Applications for CLPP Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in human heart (calculated MW of 30.2 kDa band according to NP_006003.1). IHC-P: Human liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CLPP

CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

Alternate Names

ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli), ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli), EC 3.4.21.92, Endopeptidase Clp, putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Entrez Gene IDs

8192 (Human)

Gene Symbol

CLPP

UniProt

NP_006003. (Human)

Additional CLPP Products

Product Documents for CLPP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CLPP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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