Clusterin/APOJ Antibody (PSH04-33)

Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196]

Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Western blot analysis of Clusterin/APOJ on different lysates with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: SK-Br-3 cell lysate
Lane 3: A431 cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: T-47D cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 52 kDa
Observed band size: 35-80 kDa

Exposure time: 1 minute 50 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32196) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196]

Immunohistochemistry: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/8,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32196) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.