Clusterin/APOJ Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06027
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EKALQEYRRKSRAE corresponding to C-Terminus according to NP_038520.1.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28971894).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Clusterin/APOJ Antibody
Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] -
Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] - Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western Blot: Clusterin/APOJ Antibody [NBP1-06027] -
Western Blot: Clusterin/APOJ Antibody [NBP1-06027] - (0.1ug/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Clusterin/APOJ Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.1-0.3 ug/ml
Application Notes
WB: Approx. 38kDa band observed in Mouse and Rat Brain lysates.(calculated MW of 51.7kDa according to Mouse NP_038520.2 and 51.4kDa according to Rat XP_006252156.1). This molecular weight is routinely observed by other sources and thought to correspond to the alpha chain of APOJ. Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Clusterin
Alternate Names
APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2
Gene Symbol
CLU
UniProt
Additional Clusterin Products
Product Documents for Clusterin/APOJ Antibody
Product Specific Notices for Clusterin/APOJ Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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