CMBL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82069
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVG
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%). Human reactivity reported in scientific literature (PMID: 25978681).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CMBL Antibody
Western Blot: CMBL Antibody [NBP1-82069]
Western Blot: CMBL Antibody [NBP1-82069] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissueImmunohistochemistry-Paraffin: CMBL Antibody [NBP1-82069]
Immunohistochemistry-Paraffin: CMBL Antibody [NBP1-82069] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.Western Blot: CMBL Antibody [NBP1-82069] -
Western Blot: CMBL Antibody [NBP1-82069] - Western blot analyses of ANXA3, CMBL & S100A13 expression.Depleted cyst fluids from cPTCs & benign lesions are shown together with total protein extract from A549 cells. Protein sizes are indicated to the right in kDa. Ponceau S solution used as loading control is shown below. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0126472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CMBL Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 25978681). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CMBL
Alternate Names
carboxymethylenebutenolidase homolog, carboxymethylenebutenolidase homolog (Pseudomonas), carboxymethylenebutenolidase-like (Pseudomonas), EC 3.1, FLJ23617
Gene Symbol
CMBL
Additional CMBL Products
Product Documents for CMBL Antibody
Product Specific Notices for CMBL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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