Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1A1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CMP kinase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CMPK1(NP_057392) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CMP kinase Antibody (OTI1A1) - Azide and BSA Free

Western Blot: CMP kinase Antibody (OTI1A1)Azide and BSA Free [NBP2-71947]

Western Blot: CMP kinase Antibody (OTI1A1)Azide and BSA Free [NBP2-71947]

Western Blot: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947]

Immunocytochemistry/ Immunofluorescence: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947]

Immunocytochemistry/Immunofluorescence: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CMP kinase.
Immunohistochemistry: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947]

Immunohistochemistry: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947]

Immunohistochemistry: CMP kinase Antibody (OTI1A1) - Azide and BSA Free [NBP2-71947] - Analysis of Human thyroid tissue.
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Applications for CMP kinase Antibody (OTI1A1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CMP kinase

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Alternate Names

CMKEC 2.7.4.14, CMPKUMKUMP-CMP kinase, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic, Cytidine monophosphate kinase, Cytidylate kinaseUMP/CMP kinase, Deoxycytidylate kinase, EC 2.7.4, UMP/CMPK, UMP-CMPK, UMPKUCK, Uridine monophosphate kinase, Uridine monophosphate/cytidine monophosphate kinase

Gene Symbol

CMPK1

Additional CMP kinase Products

Product Documents for CMP kinase Antibody (OTI1A1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CMP kinase Antibody (OTI1A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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