CMP kinase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38287
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMP kinase (NP_057392.1).
Sequence:
LLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
Sequence:
LLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CMP kinase Antibody
Western Blot: CMP kinase Antibody [NBP3-38287] -
Western Blot: CMP kinase Antibody [NBP3-38287] - Western blot analysis of various lysates using CMP kinase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: CMP kinase Antibody [NBP3-38287] -
Immunocytochemistry/ Immunofluorescence: CMP kinase Antibody [NBP3-38287] - Immunofluorescence analysis of MCF7 cells using CMP kinase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation: CMP kinase Antibody [NBP3-38287] -
Immunoprecipitation: CMP kinase Antibody [NBP3-38287] - Immunoprecipitation analysis of 300 ug extracts of HT-29 cells using 3 ug CMP kinase antibody. Western blot was performed from the immunoprecipitate using CMP kinase antibody at a dilution of 1:1000.Applications for CMP kinase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CMP kinase
Alternate Names
CMKEC 2.7.4.14, CMPKUMKUMP-CMP kinase, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic, Cytidine monophosphate kinase, Cytidylate kinaseUMP/CMP kinase, Deoxycytidylate kinase, EC 2.7.4, UMP/CMPK, UMP-CMPK, UMPKUCK, Uridine monophosphate kinase, Uridine monophosphate/cytidine monophosphate kinase
Gene Symbol
CMPK1
Additional CMP kinase Products
Product Documents for CMP kinase Antibody
Product Specific Notices for CMP kinase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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