Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1D6

Format

BSA Free

Concentration

1 mg/ml

View all Coactosin-like Protein 1/CotL1 Antibodies »

Product Specifications

Immunogen

Recombinant human Coactosin-like Protein 1/CotL1 (1-142aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free

Western Blot: Coactosin-like Protein 1/CotL1 Antibody (1D6)BSA Free [NBP2-31203]

Western Blot: Coactosin-like Protein 1/CotL1 Antibody (1D6)BSA Free [NBP2-31203]

Western Blot: Coactosin-like Protein 1/CotL1 Antibody (1D6) [NBP2-31203] - The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: Coactosin-like Protein 1/COTL1 Transfected 293T cell lysate
Flow Cytometry: Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free [NBP2-31203]

Flow Cytometry: Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free [NBP2-31203]

Flow Cytometry: Coactosin-like Protein 1/CotL1 Antibody (1D6) [NBP2-31203] - Analysis of Coactosin-like Protein 1/COTL1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Coactosin-like Protein 1/CotL1 Antibody (1D6) [NBP2-31203] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: PC3 cell lysateLane 2.: A549 cell lysateLane 3.: U87MG cell lysateLane 4.: HeLa cell lysate

Applications for Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coactosin-like Protein 1/CotL1

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal protein that belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified in slime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes. COTL1 is one of many proteins that participate in regulation of Actin

Alternate Names

CLP, Coactosin like Protein 1, COTL1

Gene Symbol

COTL1

Additional Coactosin-like Protein 1/CotL1 Products

Product Documents for Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Coactosin-like Protein 1/CotL1 Antibody (1D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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