COASY Antibody (2A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00080347-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COASY Antibodies »

Product Specifications

Immunogen

COASY (NP_079509, 461 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD

Reactivity Notes

Human. Other species not tested.

Specificity

COASY (2A12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for COASY Antibody (2A12)

Immunohistochemistry-Paraffin: COASY Antibody (2A12) [H00080347-M05]

Immunohistochemistry-Paraffin: COASY Antibody (2A12) [H00080347-M05]

Immunohistochemistry-Paraffin: COASY Antibody (2A12) [H00080347-M05] - Analysis of monoclonal antibody to COASY on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
ELISA: COASY Antibody (2A12) [H00080347-M05]

ELISA: COASY Antibody (2A12) [H00080347-M05]

ELISA: COASY Antibody (2A12) [H00080347-M05] - Detection limit for recombinant GST tagged COASY is approximately 1ng/ml as a capture antibody.

Applications for COASY Antibody (2A12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for immunohistochemistry (paraffin).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, pOV-2, PPAT

Entrez Gene IDs

80347 (Human)

Gene Symbol

COASY

UniProt

NP_079509 (Human)

Additional COASY Products

Product Documents for COASY Antibody (2A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COASY Antibody (2A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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