COASY Antibody (OTI3B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72224

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all COASY Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

62.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COASY Antibody (OTI3B3) - Azide and BSA Free

Western Blot: COASY Antibody (OTI3B3)Azide and BSA Free [NBP2-72224]

Western Blot: COASY Antibody (OTI3B3)Azide and BSA Free [NBP2-72224]

Western Blot: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
Immunocytochemistry/ Immunofluorescence: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224]

Immunocytochemistry/ Immunofluorescence: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224]

Immunocytochemistry/Immunofluorescence: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY.
Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224]

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224]

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Human pancreas tissue using anti-COASY mouse monoclonal antibody.
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Applications for COASY Antibody (OTI3B3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, pOV-2, PPAT

Gene Symbol

COASY

Additional COASY Products

Product Documents for COASY Antibody (OTI3B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COASY Antibody (OTI3B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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