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COBL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89615

Novus Biologicals, part of Bio-Techne
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NBP1-89615
NBP1-89615-25ul

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLT

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30866487).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COBL Antibody

Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615]

Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615]

Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615] - Staining in human parathyroid gland and smooth muscle tissues using anti-COBL antibody. Corresponding COBL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615]

Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615]

Immunohistochemistry-Paraffin: COBL Antibody [NBP1-89615] - Staining of human cerebral cortex, colon, heart muscle and smooth muscle using Anti-COBL antibody NBP1-89615 (A) shows similar protein distribution across tissues to independent antibody NBP1-89616 (B).
Western Blot: COBL Antibody [NBP1-89615]

Western Blot: COBL Antibody [NBP1-89615]

Western Blot: COBL Antibody [NBP1-89615] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-COBL antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.

Applications for COBL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COBL

Alternate Names

cordon-bleu WH2 repeat protein

Gene Symbol

COBL

Additional COBL Products

Product Documents for COBL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COBL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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