COBRA1 Antibody (F7E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29529

Novus Biologicals, part of Bio-Techne
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NBP2-29529
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # F7E4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all COBRA1 Antibodies »

Product Summary for COBRA1 Antibody (F7E4)

Immunogen

Recombinant full length human COBRA1 purified from E. coli. Accession # Q8WX92

Reactivity Notes

The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Specific for endogenous levels of the ~62 kDa COBRA1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for COBRA1 Antibody (F7E4)

Western Blot: COBRA1 Antibody (F7E4) [NBP2-29529]

Western Blot: COBRA1 Antibody (F7E4) [NBP2-29529]

Western Blot: COBRA1 Antibody (F7E4) [NBP2-29529] - Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62K COBRA1 protein.

Applications for COBRA1 Antibody (F7E4)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COBRA1

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ER-alpha) and repress ER-alpha-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)

Alternate Names

cofactor of BRCA1DKFZp586B0519, KIAA1182negative elongation factor B, NELFB, NELF-Bnegative elongation factor protein B

Entrez Gene IDs

25920 (Human)

Gene Symbol

COBRA1

UniProt

Q8WX92 (Human)

Additional COBRA1 Products

Product Documents for COBRA1 Antibody (F7E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COBRA1 Antibody (F7E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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