COBRA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15935

Novus Biologicals, part of Bio-Techne
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NBP2-15935
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COBRA1 Antibodies »

Product Summary for COBRA1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human COBRA1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (85%), Zebrafish (86%), Xenopus tropicalis (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COBRA1 Antibody

Western Blot: COBRA1 Antibody [NBP2-15935]

Western Blot: COBRA1 Antibody [NBP2-15935]

Western Blot: COBRA1 Antibody [NBP2-15935] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: COBRA1 Antibody [NBP2-15935]

Immunocytochemistry/ Immunofluorescence: COBRA1 Antibody [NBP2-15935]

Immunocytochemistry/Immunofluorescence: COBRA1 Antibody [NBP2-15935] - Immunofluorescence analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935] - Antibody detects COBRA1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. COBRA1 antibody dilution: 1:500.
View 2 more images

Applications for COBRA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COBRA1

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Alternate Names

cofactor of BRCA1DKFZp586B0519, KIAA1182negative elongation factor B, NELFB, NELF-Bnegative elongation factor protein B

Gene Symbol

COBRA1

UniProt

Q8WX92 (Human)

Additional COBRA1 Products

Product Documents for COBRA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for COBRA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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