CoCoA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21437

Novus Biologicals, part of Bio-Techne
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NBP3-21437-100ul
NBP3-21437-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CoCoA Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CoCoA fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for CoCoA Antibody

CoCoA Antibody Immunoprecipitation: CoCoA Antibody [NBP3-21437]

Immunoprecipitation: CoCoA Antibody [NBP3-21437]

CoCoA was immunoprecipitated using NBP3-21437 at 1:100 dilution, 60 ug of Immunomagnetic beads Protein A/G, secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 77 kDa. Observed band size: 100 kDa
CoCoA Antibody Immunocytochemistry/Immunofluorescence: CoCoA Antibody [NBP3-21437]

Immunocytochemistry/Immunofluorescence: CoCoA Antibody [NBP3-21437]

Immunofluorescence staining of CoCoA in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-21437 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CoCoA Antibody Immunohistochemistry-Paraffin: CoCoA Antibody [NBP3-21437]

Immunohistochemistry-Paraffin: CoCoA Antibody [NBP3-21437]

Immunochemical staining of CoCoA in human breast formalin-fixed paraffin embedded sections with NBP3-21437 at 1:300 dilution
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Applications for CoCoA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5 uL/mg of lysate

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS, pH 7.0

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CoCoA

HPS3 is involved in early stages of melanosome biogenesis and maturation. Defects in HPS3 are the cause of the cocoa (coa) mutant, and of Hermansky-Pudlak syndrome type 3 (HPS3). HPS3 is an autosomal recessive disorder, characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.

Alternate Names

calcium binding and coiled-coil domain 1, Calphoglin, Cocoa, Coiled-coil coactivator protein, coiled-coil leucine zipper coactivator 1, inorganic pyrophosphatase activator, KIAA1536calcium-binding and coiled-coil domain-containing protein 1, Sarcoma antigen NY-SAR-3

Gene Symbol

CALCOCO1

Additional CoCoA Products

Product Documents for CoCoA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CoCoA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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