Cofilin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98910

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cofilin 2 Antibodies »

Product Summary for Cofilin 2 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Cofilin 2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cofilin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody [NBP2-98910]

Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody [NBP2-98910]

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Cofilin 2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
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Applications for Cofilin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin 2

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Alternate Names

cofilin 2 (muscle), Cofilin, muscle isoform, cofilin-2, NEM7

Gene Symbol

CFL2

Additional Cofilin 2 Products

Product Documents for Cofilin 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cofilin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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