Cofilin Antibody (567)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42828

Novus Biologicals, part of Bio-Techne
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NBP2-42828
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Porcine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 567

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cofilin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin Antibody (567)

Western Blot: Cofilin Antibody (567) [NBP2-42828]

Western Blot: Cofilin Antibody (567) [NBP2-42828]

Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (567) [NBP2-42828]

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (567) [NBP2-42828]

Immunocytochemistry/Immunofluorescence: Cofilin Antibody (567) [NBP2-42828] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828]

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828]

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828] - HBL435 xenograft. Cofilin 1 antibody [567] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for Cofilin Antibody (567)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cofilin

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Alternate Names

18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18

Gene Symbol

CFL1

Additional Cofilin Products

Product Documents for Cofilin Antibody (567)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cofilin Antibody (567)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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