Cofilin Antibody (GT217)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13520

Novus Biologicals, part of Bio-Techne
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NBP3-13520
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Porcine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT217

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cofilin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cofilin Antibody (GT217)

Western Blot: Cofilin Antibody (GT217) [NBP3-13520]

Western Blot: Cofilin Antibody (GT217) [NBP3-13520]

Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.
Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520]

Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520]

Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Cofilin Antibody (GT217) [NBP3-13520]

Western Blot: Cofilin Antibody (GT217) [NBP3-13520]

Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
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Applications for Cofilin Antibody (GT217)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Alternate Names

18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18

Gene Symbol

CFL1

Additional Cofilin Products

Product Documents for Cofilin Antibody (GT217)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cofilin Antibody (GT217)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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