Cofilin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1900

Novus Biologicals, part of Bio-Techne
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NB100-1900
NB100-1900-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Microarray, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cofilin Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

Reactivity Notes

Not yet tested in other species.

Localization

Cytoplasmic and Nuclear

Specificity

Detects a band at approximately 19kDa. Reacts with muscle and non-muscle cofilin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin Antibody

Western Blot: Cofilin Antibody [NB100-1900]

Western Blot: Cofilin Antibody [NB100-1900]

Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody [NB100-1900]

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody [NB100-1900]

Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.

Applications for Cofilin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:10000
Application Notes
This antibody can be used in Western Blot. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, mouse NIH/3T3, and dog MDCK kidney cells. IF only recommended for indirect staining. Staining of cofilin is inhibited by the immunizing peptide. Also suitable for microarray. Not tested in other applications. *Optimal dilutions should be determined by the end user.

Reviewed Applications

Read 1 review rated 1 using NB100-1900 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Alternate Names

18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18

Entrez Gene IDs

1072 (Human); 12631 (Mouse); 29271 (Rat)

Gene Symbol

CFL1

UniProt

P23528 (Human)

Additional Cofilin Products

Product Documents for Cofilin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cofilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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