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Cofilin [p Ser3] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13302

Novus Biologicals, part of Bio-Techne
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NBP3-13302

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser3 of human Cofilin. The exact sequence is proprietary.

Modification

p Ser 3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cofilin [p Ser3] Antibody

Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302]

Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302]

Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin (phospho Ser3) antibody (NBP3-13302) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302]

Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302]

Western Blot: Cofilin [p Ser3] Antibody [NBP3-13302] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin (phospho Ser3) antibody (NBP3-13302) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Cofilin [p Ser3] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Alternate Names

18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18

Gene Symbol

CFL1

Additional Cofilin Products

Product Documents for Cofilin [p Ser3] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cofilin [p Ser3] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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