Cofilin [p Ser3] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23231
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 3 in human Cofilin. This sequence has 100% homology with similar regions of rat and mouse Cofilin, and has two amino acid differences from human Cofilin 2. [UniProt# P23528]
Modification
p Ser3
Specificity
This antibody was cross-adsorbed to unphosphorylated Coflin peptide then affinity purified using phospho-Cofilin (Ser-3) peptide (without carrier). The antibody detects a 19 kDa protein corresponding to the molecular mass of phosphorylated Cofilin on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Cofilin [p Ser3] Antibody
![Cofilin [p Ser3] Antibody](https://resources.bio-techne.com/images/products/nbp3-23231_rabbit-cofilin-p-ser3-pab-199202312403712.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] -")
Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] -
Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).Applications for Cofilin [p Ser3] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Cofilin
Alternate Names
18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18
Gene Symbol
CFL1
Additional Cofilin Products
Product Documents for Cofilin [p Ser3] Antibody
Product Specific Notices for Cofilin [p Ser3] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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