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Coiled-Coil Domain Containing 149 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30476

Novus Biologicals, part of Bio-Techne
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NBP2-30476
NBP2-30476-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RHQSLKKKYRELIDGDPSLPPEKRKQANLAQLLRDSQDRNKHLGEEIKELQQRLGEVQGDNKLLRMTIAKQRLGDEAIGVRHFAAHER

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Coiled-Coil Domain Containing 149 Antibody

Western Blot: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Western Blot: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Western Blot: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Immunocytochemistry/ Immunofluorescence: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Immunocytochemistry/Immunofluorescence: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Immunohistochemistry: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Immunohistochemistry: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]

Immunohistochemistry: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Staining of human kidney shows strong cytoplasmic and extracellular positivity in renal tubules.

Applications for Coiled-Coil Domain Containing 149 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coiled-Coil Domain Containing 149

Alternate Names

CCDC149, Coiled-Coil Domain-Containing Protein 149

Gene Symbol

CCDC149

UniProt

Additional Coiled-Coil Domain Containing 149 Products

Product Documents for Coiled-Coil Domain Containing 149 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Coiled-Coil Domain Containing 149 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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