coiled-coil domain containing 53 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38406
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PLNVTSVTNGAHPEATSEQPQQNSTQDSGLQESEVSAENILTVAKDPRYARYLKMVQVGVPVMAIRNKMISEGLDPDLLERPDAPVPDGESEKTVEESSDSES
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for coiled-coil domain containing 53 Antibody
Immunocytochemistry/ Immunofluorescence: coiled-coil domain containing 53 Antibody [NBP2-38406]
Immunocytochemistry/Immunofluorescence: coiled-coil domain containing 53 Antibody [NBP2-38406] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & vesicles.Immunohistochemistry: coiled-coil domain containing 53 Antibody [NBP2-38406]
Immunohistochemistry: coiled-coil domain containing 53 Antibody [NBP2-38406] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.Applications for coiled-coil domain containing 53 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: coiled-coil domain containing 53
Alternate Names
CGI-116, coiled-coil domain containing 53, Coiled-coil domain-containing protein 53, WASH complex subunit CCDC53
Gene Symbol
CCDC53
UniProt
Additional coiled-coil domain containing 53 Products
Product Documents for coiled-coil domain containing 53 Antibody
Product Specific Notices for coiled-coil domain containing 53 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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