Coilin Antibody (Pdelta) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1275
Key Product Details
Species Reactivity
Human, Mouse (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Microarray, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # Pdelta
Format
BSA Free
Concentration
1.5 mg/ml
Product Specifications
Immunogen
Recombinant fragment (C-terminus of Human coilin, 389 amino acids).
Epitope
Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.
Reactivity Notes
Cross-reacts with Human. Does not cross-react with Mouse. Not yet tested in other species.
Localization
Nuclear
Specificity
Coilin (Pdelta)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
~80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Coilin Antibody (Pdelta) - BSA Free
![Western Blot: Coilin Antibody (Pdelta)BSA Free [NB600-1275]](https://resources.bio-techne.com/images/products/Coilin-Antibody-Pdelta-BSA-Free-Western-Blot-NB600-1275-img0003.jpg "Western Blot: Coilin Antibody (Pdelta)BSA Free [NB600-1275]")
Western Blot: Coilin Antibody (Pdelta)BSA Free [NB600-1275]
Western Blot: Coilin Antibody (Pdelta) - BSA Free [NB600-1275] - HeLa cells nuclear extract were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-Coilin antibody produced in Mouse, Clone: p-delta. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat![Immunocytochemistry/ Immunofluorescence: Coilin Antibody (Pdelta) - BSA Free [NB600-1275]](https://resources.bio-techne.com/images/products/Coilin-Antibody-Pdelta-Immunocytochemistry-Immunofluorescence-NB600-1275-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Coilin Antibody (Pdelta) - BSA Free [NB600-1275]")
Immunocytochemistry/ Immunofluorescence: Coilin Antibody (Pdelta) - BSA Free [NB600-1275]
Immunocytochemistry/Immunofluorescence: Coilin Antibody (Pdelta) [NB600-1275] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-Coilin Clone: pdelta (NB600-1275). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.Applications for Coilin Antibody (Pdelta) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
WB: Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Coilin
Alternate Names
CLN80p80-coilin, coilin, coilin p80, p80
Gene Symbol
COIL
UniProt
Additional Coilin Products
Product Documents for Coilin Antibody (Pdelta) - BSA Free
Product Specific Notices for Coilin Antibody (Pdelta) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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