COL11A1 Antibody (JE40-84)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32208
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE40-84
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human COL11A1 aa 501-600 / 1,806. (Uniprot: P12107)
Localization
Secreted, extracellular space, extracellular matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for COL11A1 Antibody (JE40-84)
Western Blot: COL11A1 Antibody (JE40-84) [NBP3-32208]
Western Blot: COL11A1 Antibody (JE40-84) [NBP3-32208] - Western blot analysis of COL11A1 on different lysates with Rabbit anti-COL11A1 antibody (NBP3-32208) at 1/1,000 dilution.Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: Saos-2 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 181 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32208) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for COL11A1 Antibody (JE40-84)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COL11A1
Alternate Names
alpha-1 polypeptide, COLL6, collagen, type XI, alpha 1
Gene Symbol
COL11A1
Additional COL11A1 Products
Product Documents for COL11A1 Antibody (JE40-84)
Product Specific Notices for COL11A1 Antibody (JE40-84)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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