COL1A2 Antibody (HL2048) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25414
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2048
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for COL1A2 Antibody (HL2048) - Azide and BSA Free
Immunogen
Recombinant fragment of human COL1A2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for COL1A2 Antibody (HL2048) - Azide and BSA Free
Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -
Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -
Immunohistochemistry-Paraffin: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - COL1A2 antibody [HL2048] detects secreted COL1A2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. COL1A2 stained by COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -
Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for COL1A2 Antibody (HL2048) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COL1A2
Alternate Names
alpha 2(I)-collagen, Alpha-2 type I collagen, collagen alpha-2(I) chain, collagen I, alpha-2 polypeptide, collagen of skin, tendon and bone, alpha-2 chain, collagen, type I, alpha 2, OI4, osteogenesis imperfecta type IV, type I procollagen
Gene Symbol
COL1A2
Additional COL1A2 Products
Product Documents for COL1A2 Antibody (HL2048) - Azide and BSA Free
Product Specific Notices for COL1A2 Antibody (HL2048) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...