Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2

Format

BSA Free

Concentration

1.0 mg/ml

View all Collagen II Antibodies »

Product Specifications

Immunogen

Recombinant expressed PIIANP

Epitope

Epitope is located at the amino-terminal part of procollagen type IIA

Specificity

Procollagen type IIA N-terminal propeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Collagen II Antibody (2) - BSA Free

Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).
Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry: Collagen II Antibody (003-02) - BSA Free [NBP1-05169] - Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Positive stain of Canine Chondrocytes incubated overnight at room temperature at 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.
Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]

Immunohistochemistry: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Human chondrocytes stained with NBP1-05169 overnight at room temperature, 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.
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Applications for Collagen II Antibody (2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:1000
Application Notes
ELISA: NBP1-05169 also reacts with recombinant PIIANP coated directly onto the microtiter well. IHC: NBP1-05169 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue. Use in Canine reported by customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-05169 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Collagen II

Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Procollagen type II N-terminal propeptide (PIIANP) is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Collagen II Antibody (2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Collagen II Antibody (2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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