Collagen II Antibody (HL1772) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25418
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1772
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Collagen II Antibody (HL1772) - Azide and BSA Free
Immunogen
Recombinant fragment of human Collagen II
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Collagen II Antibody (HL1772) - Azide and BSA Free
Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -
Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -
Immunohistochemistry-Paraffin: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - Collagen II antibody [HL1772] detects secreted Collagen II protein by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. Collagen II stained by Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -
Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Collagen II Antibody (HL1772) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen II
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
Additional Collagen II Products
Product Documents for Collagen II Antibody (HL1772) - Azide and BSA Free
Product Specific Notices for Collagen II Antibody (HL1772) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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