Collagen II Antibody (M2139)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11228

Novus Biologicals, part of Bio-Techne
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NBP3-11228
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Chicken

Applications

Functional, Immunoassay, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M2139

Concentration

0.1 mg/ml

View all Collagen II Antibodies »

Product Specifications

Immunogen

The monoclonal antibody M2139 reacts with the J1 epitope (triple helical position 551-564) of collagen type II.

Epitope

J1 epitope (triple helical position 551-564) of collagen type II

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<24 EU/mg

Scientific Data Images for Collagen II Antibody (M2139)

Immunohistochemistry-Paraffin: Collagen II Antibody (M2139) [NBP3-11228]

Immunohistochemistry-Paraffin: Collagen II Antibody (M2139) [NBP3-11228]

Immunohistochemistry-Paraffin: Collagen II Antibody (M2139) [NBP3-11228] - Analysis of collagen II in paraffin-embedded mouse cartilage from paw.

Applications for Collagen II Antibody (M2139)

Application
Recommended Usage

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:25. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Collagen II

The monoclonal antibody M2139 reacts with the J1 epitope (triple helical position 551-564) of collagen type II. Collagen is a structural protein in bone, cartillage and connective tissue. Collagen type II (CII) is the major collagen of the nucleus pulposa (a component of spine), cartilage and vitreous (a component of the eye). The most commonly used animal model for rheumatoid arthritis (RA) is the collagen-induced arthritis (CIA). Transfer of collagen type II specific monoclonal antibodies induces an acute form of arthritis (collagen type II antibody induced arthritis, CAIA). The monocolonal antibody M2139 has been shown to induce CAIA in naive mice after injection of lipopolysacharide (LPS). However, in combination with the monoclonal antibody CIIC1, binding to the C1 epitope of CII, the pair of monoclonal antibodies induce arthritis in different strains of mice without any other stimulants. The presence of secondary stimulus, LPS, increases the disease incidence and severity.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Collagen II Antibody (M2139)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen II Antibody (M2139)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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