Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free

Detection of Collagen IV in Human Liver via Multiplex Immunofluorescence staining on COMET™

Collagen IV was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Detection of Collagen IV in Human Tonsil via Multiplex Immunofluorescence staining on COMET™

Collagen IV was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Detection of Collagen IV in Human Kidney via Multiplex Immunofluorescence staining on COMET™

Collagen IV was detected in immersion fixed paraffin-embedded sections of human Kidney using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.