Collagen IV Antibody (rCOL4/4742)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07596

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # rCOL4/4742

Concentration

0.2 mg/ml

View all Collagen IV Antibodies »

Product Specifications

Immunogen

Purified human placental extract

Localization

Secreted. Extracellular matrix.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08487)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Collagen IV Antibody (rCOL4/4742)

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742).
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742)

Applications for Collagen IV Antibody (rCOL4/4742)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

P29400 (Human), P53420 (Human), P08572 (Human), P02462 (Human)

Additional Collagen IV Products

Product Documents for Collagen IV Antibody (rCOL4/4742)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Collagen IV Antibody (rCOL4/4742)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.