Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Feline

Cited:

Human, Mouse, Rat, Feline

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Super-Resolution Microscopy, Western Blot

Cited:

ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Super-Resolution Microscopy, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Collagen IV Antibodies »

Product Specifications

Immunogen

Collagen IV from human and bovine placenta (Uniprot: P02462)

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Localization

Secreted and Cell surface (Potential).

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Collagen IV Antibody

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586]

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586]

Immunocytochemistry/Immunofluorescence: Collagen IV Antibody [NB120-6586] - Immunofluorescence staining of MDA-MB-231 cells for collagen IV after 7 days of culture in 6 vs. 2.5mgml^-1. Representative images of n=2 biological replicates. Scale bar 100um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29162797/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.
View 6 more images

Applications for Collagen IV Antibody

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:100

Western Blot

1:1000-1:10000
Application Notes
This product has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Use in Immunohistochemistry Free-Floating reported in scientific literature (PMID: 32245949).

Reviewed Applications

Read 7 reviews rated 4.9 using NB120-6586 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV Products

Product Documents for Collagen IV Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Collagen IV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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