Collagen V Antibody (1E2-E4/Col5) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05118CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Bovine, Canine, Chicken (Negative), Guinea Pig (Negative), Kangaroo, Mouse (Negative), Rabbit, Rat (Negative), Sheep
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E2-E4/Col5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Collagen V Antibody (1E2-E4/Col5) [CoraFluor™ 1]
Immunogen
Acid-digested pepsin soluble dog type V collagen
Specificity
Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I,
III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other
connective tissue proteins (laminin, fibronectin, elastin).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Collagen V Antibody (1E2-E4/Col5) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Collagen V
Alternate Names
alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1
Gene Symbol
COL5A1
Additional Collagen V Products
Product Documents for Collagen V Antibody (1E2-E4/Col5) [CoraFluor™ 1]
Product Specific Notices for Collagen V Antibody (1E2-E4/Col5) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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