Collagen V Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7046
![Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046]](https://resources.bio-techne.com/images/products/Collagen-V-Antibody-Immunohistochemistry-Paraffin-NB120-7046-img0002.jpg "Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046]")
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Collagen V from human and bovine placenta (Uniprot: P25940)
Reactivity Notes
This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Localization
Extracellular matrix
Specificity
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen V and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Collagen V Antibody
Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046]
Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046] - Analysis using the Biotin conjugate of NB120-7046. collagen V antibody 1:200, 45 min RT showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.
Collagen V Antibody
anti collagen V antibody ( Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WAApplications for Collagen V Antibody
Application
Recommended Usage
ELISA
1:5000-1:50000
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1:100
Western Blot
1:1000-1:10000
Application Notes
This product has been tested by dot blot and IHC and is suitable by indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27092719).
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27092719).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: Collagen V
Alternate Names
alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1
Entrez Gene IDs
1289 (Human)
Gene Symbol
COL5A1
UniProt
Additional Collagen V Products
Product Documents for Collagen V Antibody
Product Specific Notices for Collagen V Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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