Collagen VI alpha 1 Antibody (7J1P4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16869

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16869-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7J1P4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Collagen VI alpha 1 Antibodies »

Product Summary for Collagen VI alpha 1 Antibody (7J1P4)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-235 of human Collagen VI alpha 1 (P12109). DLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen VI alpha 1 Antibody (7J1P4)

Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Western Blot: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Western blot analysis of extracts of various cell lines, using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded human liver using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (7J1P4) [NBP3-16869] - Immunohistochemistry of paraffin-embedded rat lung using Collagen VI alpha 1 Rabbit mAb (NBP3-16869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Collagen VI alpha 1 Antibody (7J1P4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Collagen VI alpha 1

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Alternate Names

Collagen 6, collagen alpha-1(VI) chain, collagen VI, alpha-1 polypeptide, collagen, type VI, alpha 1, Collagen-6, human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA), OPLL

Gene Symbol

COL6A1

Additional Collagen VI alpha 1 Products

Product Documents for Collagen VI alpha 1 Antibody (7J1P4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen VI alpha 1 Antibody (7J1P4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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