Collagen VI alpha 1 Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6583

Novus Biologicals, part of Bio-Techne
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NB120-6583-50ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Feline

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Collagen VI alpha 1 Antibodies »

Product Summary for Collagen VI alpha 1 Antibody [Biotin]

Immunogen

Collagen VI alpha 1 from human and bovine placenta (Uniprot: P12109)

Reactivity Notes

This antibody reacts with most mammalian Collagen VI alpha 1 and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Feline reactivity reported in scientific literature (PMID: 33091431).

Localization

Extracellular matrix.

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen VI alpha 1 and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Scientific Data Images for Collagen VI alpha 1 Antibody [Biotin]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [Biotin] [NB120-6583] - Collagen VI Antibody [Biotin] [NB120-6583] - collagen VI antibody 1:400 45 min RT showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.

Applications for Collagen VI alpha 1 Antibody [Biotin]

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:5000-1:10000
Application Notes
This product is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen VI alpha 1

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Alternate Names

Collagen 6, collagen alpha-1(VI) chain, collagen VI, alpha-1 polypeptide, collagen, type VI, alpha 1, Collagen-6, human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA), OPLL

Gene Symbol

COL6A1

UniProt

P12109 (Human)

Additional Collagen VI alpha 1 Products

Product Documents for Collagen VI alpha 1 Antibody [Biotin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen VI alpha 1 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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