Collagen VII Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00174

Novus Biologicals, part of Bio-Techne
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NBP1-00174
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Canine (100%), Human (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Collagen VII Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TVQYSDDPRTEF corresponding to internal region according to NP_000085.1.

Specificity

This product does not work on cryostat sections of Normal Human Skin.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Collagen VII Antibody

Western Blot: Collagen VII Antibody [NBP1-00174]

Western Blot: Collagen VII Antibody [NBP1-00174]

Western Blot: Collagen VII Antibody [NBP1-00174] - Analysis of Collagen VII in rat testis lysate (35ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Collagen VII Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 1.5 ug/ml
Application Notes
An anonymous customer has reported unsuccessful usage on cryosections of human Skin. WB: Approx. 250 kDa band observed in rat testis lysates (calculated MW of 159 kDa band according to rat NP_001100328.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Collagen VII

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq]

Alternate Names

Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen

Entrez Gene IDs

1294 (Human); 12836 (Mouse); 301012 (Rat)

Gene Symbol

COL7A1

UniProt

NP_000085.1 (Human)

Additional Collagen VII Products

Product Documents for Collagen VII Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen VII Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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