Collagen XVII Antibody (8W2C8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16485
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8W2C8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human Collagen XVII (Q9UMD9). SVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Collagen XVII Antibody (8W2C8)
![Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485]](https://resources.bio-techne.com/images/products/Collagen-XVII-Antibody-8W2C8-Western-Blot-NBP3-16485-img0002.jpg "Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485]")
Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485]
Western Blot: Collagen XVII Antibody (8W2C8) [NBP3-16485] - Western blot analysis of extracts of A-431 cells, using Collagen XVII Rabbit mAb (NBP3-16485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: Collagen XVII Antibody (8W2C8) [NBP3-16485]](https://resources.bio-techne.com/images/products/Collagen-XVII-Antibody-8W2C8-Immunohistochemistry-Paraffin-NBP3-16485-img0001.jpg "Immunohistochemistry-Paraffin: Collagen XVII Antibody (8W2C8) [NBP3-16485]")
Immunohistochemistry-Paraffin: Collagen XVII Antibody (8W2C8) [NBP3-16485]
Immunohistochemistry-Paraffin: Collagen XVII Antibody (8W2C8) [NBP3-16485] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Collagen XVII Rabbit mAb (NBP3-16485) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Collagen XVII Antibody (8W2C8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Collagen XVII
Long Name
Collagen Type XVII Alpha 1
Alternate Names
BP180, BPA-2, BPAG2, COL17A1, ERED, LAD-1
Gene Symbol
COL17A1
Additional Collagen XVII Products
Product Documents for Collagen XVII Antibody (8W2C8)
Product Specific Notices for Collagen XVII Antibody (8W2C8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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