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Collectrin/TMEM27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13450

Novus Biologicals, part of Bio-Techne
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NBP2-13450
NBP2-13450-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: LVTAIHAELCQPGAENAFKVRLSIRTALGDKAYAWDTNEEYLFKAMVAFSMRKVPNREATEISHVLLCNVTQRVSFWFVVTDPSKNHTLPAVEVQSAIRMNKNRINNAFFLNDQTLE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collectrin/TMEM27 Antibody

Immunocytochemistry/ Immunofluorescence: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunocytochemistry/ Immunofluorescence: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunocytochemistry/Immunofluorescence: Collectrin/TMEM27 Antibody [NBP2-13450] - Immunofluorescent staining of human cell line HaCaT shows localization to endoplasmic reticulum & vesicles.
Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450] - Staining in human kidney and lymph node tissues using anti-TMEM27 antibody. Corresponding TMEM27 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450]

Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450] - Staining of human kidney shows high expression.

Applications for Collectrin/TMEM27 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collectrin

The Tmem27 gene encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brushborder of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of theSNARE (soluble N-ethylm

Alternate Names

NX17, TMEM27

Gene Symbol

CLTRN

Additional Collectrin Products

Product Documents for Collectrin/TMEM27 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Collectrin/TMEM27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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