COMMD8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82178
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for COMMD8 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SRKDEIKQALSREIVAISSAQLQDFDWQVKLALSSDKIAALRMPLLSLHLDVKENGEVKPYSIEMSREELQNLIQSLEAANKVVLQLK
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COMMD8 Antibody
Western Blot: COMMD8 Antibody [NBP1-82178]
Western Blot: COMMD8 Antibody [NBP1-82178] - Analysis in control (vector only transfected HEK293T lysate) and COMMD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: COMMD8 Antibody [NBP1-82178]
Immunocytochemistry/Immunofluorescence: COMMD8 Antibody [NBP1-82178] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.Immunohistochemistry-Paraffin: COMMD8 Antibody [NBP1-82178]
Immunohistochemistry-Paraffin: COMMD8 Antibody [NBP1-82178] - Staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.Applications for COMMD8 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COMMD8
Alternate Names
COMM domain containing 8, COMM domain-containing protein 8, FLJ20502
Gene Symbol
COMMD8
Additional COMMD8 Products
Product Documents for COMMD8 Antibody
Product Specific Notices for COMMD8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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