Common gamma Chain/IL-2 R gamma Antibody (3E12) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12038
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Common gamma Chain/IL-2 R gamma Antibody (3E12) - Chimeric - Azide and BSA Free [NBP3-12038]](https://resources.bio-techne.com/images/products/Common-gamma-Chain-IL-2-R-gamma-Antibody-3E12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12038-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Common gamma Chain/IL-2 R gamma Antibody (3E12) - Chimeric - Azide and BSA Free [NBP3-12038]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 3E12
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).
Specificity
This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognises an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: Common gamma Chain/IL-2 R gamma Antibody (3E12) - Chimeric - Azide and BSA Free [NBP3-12038]
Immunocytochemistry/Immunofluorescence: Common gamma Chain/IL-2 R gamma Antibody (3E12) - Chimeric [NBP3-12038] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 3E12 (NBP3-12038) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12038, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Common gamma Chain/IL-2 R gamma
Long Name
IL-2 Receptor gamma
Alternate Names
CD132, IL-2 R gamma, IL2R gamma, IL2RG
Gene Symbol
IL2RG
Additional Common gamma Chain/IL-2 R gamma Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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