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COMP/Thrombospondin-5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92733

Novus Biologicals, part of Bio-Techne
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NBP2-92733-0.02ml
NBP2-92733-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for COMP/Thrombospondin-5 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2). GQGQSPLGSDLGPQMLRELQETNAALQDVRELLRQQVREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGVACIQTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COMP/Thrombospondin-5 Antibody - BSA Free

Western Blot: COMP/Thrombospondin-5 AntibodyBSA Free [NBP2-92733]

Western Blot: COMP/Thrombospondin-5 AntibodyBSA Free [NBP2-92733]

Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: COMP/Thrombospondin-5 Antibody - BSA Free [NBP2-92733]

Immunocytochemistry/ Immunofluorescence: COMP/Thrombospondin-5 Antibody - BSA Free [NBP2-92733]

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of L929 cells using COMP Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
COMP/Thrombospondin-5 Antibody

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] -

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of Human Dermal Fibroblasts (HDFa). Green: 1:100 COMP/Thrombospondin-5 antibody in PBS/BSA 1%, 1h room temperature Blue: DAPI. Image from verified customer review.

Applications for COMP/Thrombospondin-5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-92733 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for COMP/Thrombospondin-5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COMP/Thrombospondin-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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