Complement C3a Antibody (6E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17998

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E8

Concentration

1 mg/ml

View all Complement C3a Antibodies »

Product Specifications

Immunogen

Recombinant netrin domain of human C3. UniProt: P01024

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Complement C3a Antibody (6E8)

Western Blot: Complement C3a Antibody (6E8) [NBP3-17998]

Western Blot: Complement C3a Antibody (6E8) [NBP3-17998]

Western Blot: Complement C3a Antibody (6E8) [NBP3-17998] - Western blot analysis of Complement C3a antibody (6E8) on human serum samples. 0.1ug purified human C3 protein (lane 1) and 10 ug normal human serum protein (lane 2) were blotted with Complement C3a antibody (6E8) at a concentration 1:3000. The Complement C3a antibody (6E8) monoclonal binds strongly and cleanly to a band at about 115 kDa which represents the intact alpha subunit of C3 and a weaker proteolytic band at approximately 40kDa. Bands at 190kDa and above are likely the pro-C3 and its glycosylated form.

Applications for Complement C3a Antibody (6E8)

Application
Recommended Usage

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C3a

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1). C3 is cleaved by C3 convertase into two fragments, the smaller C3a and larger C3b (1,2). Human C3a is 77 amino acids (aa) in length and has a theoretical molecular weight of ~9 kDa (2). Structurally, complement C3a consists of four anti-parallel helical structures with three disulfide bridges (2). The C3a fragment binds the G-protein coupled receptor (GPCR) C3a (C3aR) (2,3). Functionally, C3a plays an important role in regulating both adaptive immunity and innate immunity (1-3).

The C3a/C3aR pathway is often referred to as a "double-edged sword" because it has both anti-inflammatory and pro-inflammatory functions (2,3). For instance, its anti-inflammatory role includes pathogen elimination and reduced interferon-gamma (IFN-gamma) expression and cytotoxicity in natural killer cells (2). By contrast, complement C3a is an anaphylatoxin and plays a role in inflammation by prompting histamine release from mast cells and increasing permeability of the vascular system (2-4). The C3a/C3aR pathway has also been indicated in a number of diseases including Alzheimer's disease, asthma, and kidney disease (3). Patients with various kidney diseases tend to show higher levels of complement C3a in the plasma and urine (3).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Gao, S., Cui, Z., & Zhao, M. H. (2020). The Complement C3a and C3a Receptor Pathway in Kidney Diseases. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01875

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Haddad, A., & Wilson, A. M. (2020). Biochemistry, Complement. In StatPearls. StatPearls Publishing.

Alternate Names

Acylation Stimulating Protein, acylation-stimulating protein cleavage product, AHUS5, ARMD9, ASP, C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a, C3a anaphylatoxin, C3adesArg, C3b, C3bc, C3-beta-c, complement C3, Complement C3 alpha chain, Complement C3 beta chain, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3c alpha' chain fragment 2, Complement C3d fragment, Complement C3dg fragment, Complement C3f fragment, Complement C3g fragment, complement component 3, complement component C3, complement component C3a, complement component C3b, CPAMD1, EC 3.4.21.43, epididymis secretory sperm binding protein Li 62p, HEL-S-62p, prepro-C3

Gene Symbol

C3

Additional Complement C3a Products

Product Documents for Complement C3a Antibody (6E8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement C3a Antibody (6E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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