Complement Component C1qA Antibody (JL-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51139

Novus Biologicals, part of Bio-Techne
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NBP1-51139
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Conjugate
Catalog #
Biotin
NBP1-51140B
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Functional, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # JL-1

Concentration

0.1 mg/ml

View all Complement Component C1qA Antibodies »

Product Specifications

Immunogen

Purified mouse C1QA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Positive Control: Spleen and kidney tissue of wild-type mice (Ref.1) Negative control: Spleen and kidney tissue of C1q -/- mice (Ref.1)

Scientific Data Images for Complement Component C1qA Antibody (JL-1)

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139]

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139]

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139] - Intracellular flow cytometry for C1q in peritoneal macrophages. Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions. This is image was submitted via customer review.

Applications for Complement Component C1qA Antibody (JL-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-51139 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Component C1qA

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

Long Name

Complement Component 1q Subcomponent, A Chain

Alternate Names

C1QA

Gene Symbol

C1QA

Additional Complement Component C1qA Products

Product Documents for Complement Component C1qA Antibody (JL-1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement Component C1qA Antibody (JL-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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