Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9

Format

BSA Free

Concentration

1.0 mg/ml

View all Complement Component C1s Antibodies »

Product Specifications

Immunogen

C1s proenzyme purified from human plasma

Specificity

Specific for C1s proenzyme and activated free C1s

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

Immunohistochemistry: Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free [NBP1-05023]

Immunohistochemistry: Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free [NBP1-05023]

Immunohistochemistry: Complement Component C1s Antibody (C1s9) - (Pro and Active) - BSA Free [NBP1-05023] - Plasma shows distinct positivity stained with Complement Component C1s Antibody.

Applications for Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

Application
Recommended Usage

ELISA

1:20000

Immunohistochemistry

1:10-1:500
Application Notes
ELISA: NBP1-05023 reacts in ELISA when tested with activated C1s enzyme directly coated onto the microtiter well or in sandwich ELISA in combination with a polyclonal antibody in coat.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Complement Component C1s

C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes (1) but also by other cells (2,3). The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2 (1,4,5).

Alternate Names

C1S

Gene Symbol

C1S

Additional Complement Component C1s Products

Product Documents for Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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