Complement Component C1s Antibody (M241)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52122

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Flow Cytometry, Immunoassay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M241

Concentration

0.1 mg/ml

View all Complement Component C1s Antibodies »

Product Specifications

Immunogen

The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s.

Specificity

The antibody binds specifically to the active centre of C1s.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Complement Component C1s Antibody (M241)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS containing 0.1% bovine serum albumin.

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Component C1s

Monoclonal antibody M241 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.

Alternate Names

C1S

Entrez Gene IDs

716 (Human); 716 (Human)

Gene Symbol

C1S

Additional Complement Component C1s Products

Product Documents for Complement Component C1s Antibody (M241)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Complement Component C1s Antibody (M241)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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